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The chemical methods for preparation of gold nanoparticles lead to the presence of some toxic chemicals adsorbed on the surface that may have adverse effects in their medical applications. Hence, the need to develop environmentally benign nanoparticles has growing interest. Aqueous extract of Baker’s yeast (Saccharomyces cerevisiae) has been used for biosynthesis of gold nanoparticles with 13.0±0.9nm size under visible light. The existed components in the extract were identified as the following; trimethylsilyl derivatives of butan-2,3-diol, glucose, indole-3-acetic acid and undecanoic acid could be act as capping and reducing agents for gold nanoparticles. The cytotoxicity of the biosynthesized gold nanoparticles, chemically synthesized gold nanoparticles with ~5.0±2.0nm size and aqueous extract of Baker’s yeast towards Ehrlich ascites carcinoma cancer cells has been studied using Trypan blue exclusion method. The results show that the killed percentage of Ehrlich ascites carcinoma cells under dark incubation of aqueous extract of Baker’s yeast, chemically synthesized gold nanoparticles and the photo-biosynthesized gold nanoparticles are 9.7%, 12.5% and 24.6 %, respectively. These percentages have been increased to 10.63%, 60.6% and 86.5% under visible light incubation, respectively. The enhancement of the Ehrlich ascites carcinoma cells killing under visible light incubation is proposed based on the photothermal properties of the formed plasmonic gold nanoparticles that conjugated with anti-epidermal growth factor receptor antibodies. In case of the photo-extracellular synthesized gold nanoparticles sample, cancer cells death percentage also has been induced by the yeast phagocytosis into the cancer cell and thereby induction of cancer cell apoptosis. We have been observed that the combination between two different treatment ways can give a third one with high affinity on the cancer disease treatment.